PROJECT TITLE :

Three Phoma spp. synthesised novel silver nanoparticles that possess excellent antimicrobial efficacy

ABSTRACT:

The authors report extracellular mycosynthesis of silver nanoparticles (AgNPs) by Phoma capsulatum, Phoma putaminum and Phoma citri. The AgNPs therefore synthesised were characterised by UV-visible spectrophotometer, Fourier remodel infrared spectroscopy, Nanosight LM20 and transmission electron microscopy, which confirmed the synthesis of mostly spherical and polydisperse nanoparticles capped with proteins. The dimensions of AgNPs was found within the range of ten-80 , five-eighty and five-ninety nm with a mean size of 31.eighty five, twenty five.forty three and 23.29 nm by P. capsulatum, P. putaminum and P. citri, respectively. Any, potential antimicrobial activity was reported against Aspergillus niger, Candida albicans, Salmonella choleraesuis, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The lowest minimal inhibitory concentration (MIC) (0.85 μg/ml) was reported for AgNPs synthesised from P. citri against S. choleraesuis. However, AgNPs synthesised from P. capsulatum showed the best MIC (ten.sixty two μg/ml) against S. choleraesuis, P. aeruginosa and E. coli (clinical isolate). The same MIC values (10.sixty two μg/ml) were also reported against P. aeruginosa and both clinical and customary isolates of E. coli for AgNPs synthesised from P. citri. It was additionally observed that each one the silver nanoparticles showed remarkable antifungal and antibacterial activity against these tested pathogens as compared with the commercially out there antifungal and antibacterial agents.